K.K. DNAFORM


C. elegans RNAi Library

C. elegans has proved invaluable as a model system for studies in developmental biology and beyond. The Caenorhabditis elegans RNAi feeding library from Julie Ahringer's group at the The Wellcome CRC Institute, University of Cambridge, Cambridge, England, opens up new possibilities for studying gene functions in C. elegans.


C. elegans genomic fragments were PCR amplified using Research Genetics GenePairs, cloned into the EcoRV site of vector L4440 from Timmons and Fire, and transformed into bacterial strain HT115 as described (Nature 408, 325-330). The whole genome library consists of 16,757 bacterial strains, which cover 87% of C. elegans genes.


Note: Bacterial strains carry the GenePairs name, which may not correspond to a predicted C. elegans gene name.


A current mapping of GenePair to gene can be found in WormBase.
http://www.wormbase.org/
GenePairs primer sequences are available at
http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html
The complete C. elegans RNAi database can be downloaded by under
http://www.geneservice.co.uk/products/rnai/datasheets/c_elegans_chrom_all.txt

Format

DNAFORM offers RNAi libraries by individual chromosome sets (I, II, III, IV, V and X) as frozen glycerol stocks of bacterial strains arrayed in 384 well plates or as individual bacterial strains (clones).


  • Chromosome sets I : 2,445clone (384Plate ×8)
  • Chromosome sets II : 2,974clone (384Plate ×9)
  • Chromosome sets III : 2,132clone (384Plate ×7)
  • Chromosome sets IV : 2,693clone (384Plate ×8)
  • Chromosome sets V : 3,656clone (384Plate ×13)
  • Chromosome sets X : 2,356clone (384Plate ×7)
  • Total : 16,256clone (384Plate ×52)


We are also supplying re-arrayed sub-sets of the libraries in the following areas.
各セットの詳細はリンク先のデータシート(Excelファイル)をご参照下さい。



For further information contact us under.
Contact Form

Links

More information about the C. elegans RNAi Library can be found at the homepage of the Source BioScience under the following links.

C. elegans RNAi Database

References

  • Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, and Ahringer J.: “Functional genomic analysis of C. elegans chromosome I by systematic RNA interference.” Nature. 2000 Nov 16;408(6810):325-30. Wellcome/CRC Institute, University of Cambridge, UK.