K.K. DNAFORM


Custom Eprobe®/Eprimer™ Synthesis

What can Eprobe®/Eprimer™ Offer You?

Eprobe®/Eprimer™ is a fluorescence labeled oligonucleotide that provides target-specific signals with significant signal to noise ratio. At the single-strand state, the two fluorescence dyes attached to Eprobe®/Eprimer™ are quenched by interaction of each other. Upon hybridation to a target template, the dyes are separated and emits a strong fluorescence signal.

DNAFORM provides custom Eprobe®/Eprimer™ for your research.

Benefits of Using Eprobe®/Eprimer™

  • High S/N ratio
  • High target specifity
  • Real-time PCR and melting curve analysis with a single probe

Applications of Eprobe® PCR

  • Real-time PCR
  • SmartAmp® and other isothermal amplification methods
  • Melging curve analysis

Specifications

Quality and Consistency

  • Guaranteed yield: 10 nmol
  • HPLC purification
  • Maximum length: 26 bases

Distribution

The products is shipped as a dried product in a 1.5 mL micro tube.

Instructions

  • Currently, fluorescent-dye-labeling at a thymidine (T) site is only available.
  • Multi-labeling is not available.
  • Thiazole yellow (EX/EM: 430/450 nm), Thiazole orange (EX/EM: 510/530 nm) and Thiazole pink (EX/EM: 570/590 nm) are available as a dye.
  • 3'-end labeling is not available
  • Avoid labeling at nucleotides positioned less than 3nt from 5'-end or 3'-end. It may cause undesired affection in reaction/detection due to change in colour, increased baseline signal, and other unpredictable effects on the labeled oligonucleotide.
    Ex.: 5'-ZAGCAGC..., 5'-AZCCGCA..., 5'-AGZCGA..., ...AGCZGC-3', ...CGAZG-3'
    (Z: dye-labeled T)
  • Maximum allowable length of a mononucleotide repeat is four (for example ...GGGG...), otherwise we cannot guarantee minimum yield 10 nmol.
  • Contact us if you need consultation about the dye position.

Long Oligos Up to 50 Bases (consultation required)

Large scale, guaranteed yield: 10 nmol

Maximum 50 bases

Quality Control

Purity assay with reverse phase HPLC (all products, minimum 80% purity)

Purity assay with PAGE (sampling test)


Shipping Schedule

Products 3' modification Dye (EX/EM) Shipping
Eprobe® 3'-Spacer C3 Thiazole yellow (430/450) 6 business days
Eprobe® 3'-Spacer C3 Thiazole orange (510/530) 6 business days
Eprobe® 3'-Spacer C3 Thiazole pink (570/590) 11 business days
Eprimer™ - Thiazole yellow (430/450) 6 business days
Eprimer™ - Thiazole orange (510/530) 6 business days
Eprimer™ - Thiazole pink (570/590) 11 business days


*Thiazole orange attached oligonucleotide ordered before 5 p.m. E.T., Friday is shipped on Monday after next week.

*Thiazole orange attached oligonucleotide ordered before 5 p.m. E.T., Friday is shipped on Tuesday after 3 weeks.

*Shipping may extend by order situation.


Notes

  • This product is intended for research purposes only.
  • DNAFORM and RIKEN have acquired joint patent of this product.
  • This product is currently not available outside Japan.


Related products