Drosophila Gene Collection (Berkeley)
□ Drosophila Gene Collection (Berkeley)
The Berkeley Drosophila Genome Project (BDGP), a consortium of the Drosophila Genome Center, has produced over 240,000 ESTs derived from various tissues. The goal of the consortium includes full-insert sequencing and assembly of non-redundant cDNA clones. Annotated sequences of all Drosophila transcripts, including products of alternative splicing, will facilitate creation of a set of Drosophila Gateway clones for use in proteomics and other functional genomics projects.
A set of ~11,000 clones has been assembled from ~90,000 EST sequences, grouped or clotted, based on sequence identity from their 5' ends. One representative from each clot was rearrayed and its 5' and 3' ends sequenced, first to verify its identity, and second to recluster on the basis of the 3' sequence.
DGeneration of the Release 3 annotation of the genome made extensive use of our full-insert sequence data. In the course of that effort, human curators identified a total of 1,860 clones that have become the DGCr3. The DGCr3 currently includes clones chosen to replace clones with truncated ORFs, clones for genes that are not currently represented in the DGC, plus clones that represent putative alternative splicing forms.
The clonese from the Drosophila Gene Collection is provided as a E. coli glycerol stock. DNAFORM offeres the resources as a indivisual clone, an indivisual 386-well plaset format. Larger optional (39 plates; DGCr 1.0 = 17 plates, DGCr 2.0 = 16 plates, DGCr 3.0 = 6plateds) is available as well.
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- Stapleton M et al: A Drosophila full-length cDNA resource. Genome Biol. 2002;3(12):RESEARCH0080. Epub 2002 Dec 23.