Rat Full-Length cDNA Clones : Technology Overview
□ Technical Information
DNAFORM has prepared a number of full-length enriched cDNA libraries from the rat strain F344, which are available for sales.
The cDNA libraries were prepared applying the patented RIKEN/DNAFORM full-length cDNA cloning technology assuring full-length rate as high as up to 90 to 95%. cDNA inserts were cloned into the phage λ-FLC-III, which offer a wide cloning range from 500 to 15,000 bp. In addition our phage/vector system allows for the excision of the plasmid vector from the phages by the Cre-loxP recombination system, and uses homing restriction endonucleases for a safe release of the entire inserts omitting the risk of internal cleavage.