Rat Full-Length cDNA Clones
DNAFORM has prepared a number of full-length enriched cDNA libraries from the rat strain F344, which are available for sales.
The cDNA libraries were prepared applying the patented RIKEN/DNAFORM full-length cDNA cloning technology assuring full-length rate as high as up to 90 to 95%. cDNA inserts were cloned into the phage λ-FLC-III, which offer a wide cloning range from 500 to 15,000 bp. In addition our phage/vector system allows for the excision of the plasmid vector from the phages by the Cre-loxP recombination system, and uses homing restriction endonucleases for a safe release of the entire inserts omitting the risk of internal cleavage.
- □ One time amplification Phage DMSO stock ( >1x 106 pfu/μl )
- □ Plasmid glycerol stock ( >1x 105 cfu )
□Shipment and Storage
Libraries are shipped on dry ice. DMSO and Glycerol stocks should be stored at -80°C.
□Quality Check before Delivery
- □ Insert size analysis (max. 24 clones)
- □ Sequence analysis of cDNA 5’end (max. 24 clones)
□List of the Rat Full-Length cDNA Library
|Rat001||Liver||Adult+Embryo (20 days)||Normalized Library (2 pooling)|
|Rat002||Kidney||Adult+Embryo (20 days)||Normalized Library (2 pooling)|
|Rat003||Spleen||Adult+Embryo (20 days)||Normalized Library (2 pooling)|
|Rat004||Colon/Intestine||Adult：Colon Embryo:Intestine||Normalized Library (2 pooling)|
|Rat009||Liver||Embryo (20 days)||Standard|
|Rat014||Skin||6 weeks||Normalized Library|
* Libraries are offered for research purpose only;
not for use in human or animals; not for use in therapy or diagnostics.