Rat Full-Length cDNA Clones
□Introduction
DNAFORM has prepared a number of full-length enriched cDNA libraries from the rat strain F344, which are available for sales.
The cDNA libraries were prepared applying the patented RIKEN/DNAFORM full-length cDNA cloning technology assuring full-length rate as high as up to 90 to 95%. cDNA inserts were cloned into the phage λ-FLC-III, which offer a wide cloning range from 500 to 15,000 bp. In addition our phage/vector system allows for the excision of the plasmid vector from the phages by the Cre-loxP recombination system, and uses homing restriction endonucleases for a safe release of the entire inserts omitting the risk of internal cleavage.
□Delivery
- □ One time amplification Phage DMSO stock ( >1x 106 pfu/μl )
- □ Plasmid glycerol stock ( >1x 105 cfu )
□Shipment and Storage
Libraries are shipped on dry ice. DMSO and Glycerol stocks should be stored at -80°C.
□Quality Check before Delivery
- □ Insert size analysis (max. 24 clones)
- □ Sequence analysis of cDNA 5’end (max. 24 clones)
□List of the Rat Full-Length cDNA Library
Cat. # | Tissues | Stage | Specification |
---|---|---|---|
Rat001 | Liver | Adult+Embryo (20 days) | Normalized Library (2 pooling) |
Rat002 | Kidney | Adult+Embryo (20 days) | Normalized Library (2 pooling) |
Rat003 | Spleen | Adult+Embryo (20 days) | Normalized Library (2 pooling) |
Rat004 | Colon/Intestine | Adult:Colon Embryo:Intestine | Normalized Library (2 pooling) |
Rat005 | Thymus | Adult | Normalized Library |
Rat006 | Lung | Adult | Normalized Library |
Rat007 | Brain | Adult | Normalized Library |
Rat008 | Kidney | Adult | Normalized Library |
Rat009 | Liver | Embryo (20 days) | Standard |
Rat010 | Testis | Adult | Standard |
Rat011 | Colon | Adult | Standard |
Rat012 | Kidney | Adult | Standard |
Rat013 | Skin | 6 weeks | Standard |
Rat014 | Skin | 6 weeks | Normalized Library |
Rat015 | Muscle | Adult | Normalized Library |
* Libraries are offered for research purpose only;
not for use in human or animals; not for use in therapy or diagnostics.