Description
DNAFORM has prepared a number of full-length enriched cDNA libraries from the rat strain F344, which are available for sales.
The cDNA libraries were prepared applying the patented RIKEN/DNAFORM full-length cDNA cloning technology assuring full-length rate as high as up to 90 to 95%. cDNA inserts were cloned into the phage λ-FLC-III, which offer a wide cloning range from 500 to 15,000 bp. In addition our phage/vector system allows for the excision of the plasmid vector from the phages by the Cre-loxP recombination system, and uses homing restriction endonucleases for a safe release of the entire inserts omitting the risk of internal cleavage.
Delivery
One time amplification Phage DMSO stock |
( >1x 106 pfu/µl ) |
Plasmid glycerol stock |
( >1x 105 cfu ) |
Shipment and Storage
Libraries are shipped on dry ice. DMSO and Glycerol stocks should be stored at -80°C.
Quality Check before Delivery
Insert size analysis (max. 24 clones)
Sequence analysis of cDNA 5’end (max. 24 clones)
Additional Services on clone isolation are available on request!
Available Rat Full-Length cDNA Libraries
| Cat. # |
Tissues |
Stage |
Specification |
Rat001 | Liver | Adult + Embryo (20 days) | Normalized Library (2 pooling) |
Rat002 | Kidney | Adult + Embryo | Normalized Library (2 pooling) |
Rat003 | Spleen | Adult + Embryo | Normalized Library (2 pooling) |
Rat004 | Colon/Intestine | Adult :Colon
Embryo:Intestine | Normalized Library (2 pooling) |
Rat005 | Thymus | Adult | Normalized Library |
Rat006 | Lung | Adult | Normalized Library |
Rat007 | Brain | Adult | Normalized Library |
Rat008 | Kidney | Adult | Normalized Library |
Rat009 | Liver | Embryo | Standard Library |
Rat010 | Testis | Adult | Standard Library |
Rat011 | Colon | Adult | Standard Library |
Rat012 | Kidney | Adult | Standard Library |
Rat013 | Skin | 6 weeks | Standard |
Rat014 | Skin | 6 weeks | Normalized |
Rat015 | Muscle | Adult | Normalized |
Price List
| Form |
Item |
Price |
A |
One time amplification Phage DMSO stock
Plasmid glycerol stock
Analysis data of cDNA insert size and 5’end sequence |
JPY600,000 |
B |
Plasmid glycerol stock
Analysis data of cDNA insert size and 5’end sequence |
JPY450,000 |
C |
One time amplification Phage DMSO stock |
JPY300,000 |
Prices do not include shipping cost.
DNAFORM full-length rat cDNA libraries are not available for sales in the USA.
Libraries are offered for research purpose only;
not for use in human or animals; not for use in therapy or diagnostics.
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