ORF Clones: Clone Information

DNAFORM provides ORF Clones in two formats:

For ORF clones ordered as "Plasmid DNA", DNAFORM ships 50ng purified plasmid DNA. The lyophilized DNA is stable at room temperature. After dissolving the DNA in TE buffer or alike, the DNA solution should be stored at -20°C.

For ORF clones ordered as "Slant tube", DNAFORM ships living bacteria on agar. Upon arrival, the culture should be stored at 4°C. Note a bacterial culture cannot be stored for an extended period of time. Therefore we advise you to plate the bacteria on a LB agar plate with the appropriate antibiotic as soon as possible and to grow a new culture. It is preferable to select some individual clones and to check their inserts before using the clone(s) for further studies.

NOTE, ORF clones are provided on different vectors and do require different selection media with different anibiotics Reger to the datasheet for more information on which amibibiotic to use for your ORF clones.

Lid colour	Antibiotic	Concentration
Yellow	Kanamycin	10μg/ml
Purple	Kanamycin	25μg/ml
Clear	Spectinomycin	100μg/ml

Plasmid Name	Antibiotic	Vector map
pENTR223.1	Spectinomycin resistant; 100 μg/ml	PDF
pENTR223	Spectinomycin resistant; 100 μg/ml	PDF
pENTR221	Kanamycin resistant; 10 μg/ml	PDF
pENTR201	Kanamycin resistant; 10 μg/ml	PDF
pENTR/D-TOPO	Kanamycin resistant; 25 μg/ml	PDF
pENTR223.1-LC	Spectinomycin resistant; 100 μg/ml	PDF
pDONR223	Spectinomycin resistant; 100 μg/ml	PDF

More information about the ORF project and ORF clones can be found at the homepage of the ORFeome Collaboration under the following links:

ORFeom library information gives information about the library including flanking sequences:
> http://www.orfeomecollaboration.org/html/humlib_info.shtml

An sequence-based search tool for the ORF collection can be found at:
> http://www.orfeomecollaboration.org/OQ/

Hartley, J.L., Temple, G.F. and Brasch, M.A. (2000) DNA cloning using in vitro site-specific recombination. Genome Res, 10, 1788-95.

Rual, J.F., Hirozane-Kishikawa, T., Hao, T., Bertin, N., Li, S., Dricot, A., Li, N., Rosenberg, J., Lamesch, P., Vidalain, P.O. et al. (2004) Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res, 14, 2128-35.

Lamesch, P., Li, N, Milstein, S et al. (2006) hORFeome v3.1: A Resource of Human Open Reading Frames Covering over 10,000 Human Genes. In preparation