NIH Drosophila cDNA Library

This library was constructed by Brian Oliver at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size-fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. Clones are provided in the vector pBluescript SK (-) and the host strain is E.coli SOLR.

DNAFORM can offer approximately 3930 clones from plates (labelled bs) numbered 3- 24, 26- 37, 40- 42, 44- 46 and 49. For all plates except bs49 double-spotted high-density filter for screening by hybridisation are available on request. For further information contact us under:dna@dnaform.jp.

1. http://intramural.niddk.nih.gov/research/faculty.asp?People_ID=1526
2. http://flybase.bio.indiana.edu/