Following on from the success of the C. elegans ORFeome library, Marc Vidal and
his lab, has given us access to distribute his C. elegans Promoterome library. The
emerging field of systems biology requires tools for promoter studies to link the
expression of genes to regulatory pathways. The promoter library includes clones
containing ~6,000 predicted promoters, cloned into the highly flexible MultiSite
Gateway™ system (Invitrogen). These promoters can be transferred easily into
various Gateway™ Destination vectors to drive expression of markers such as GFP
alone (promoter::GFP constructs), or in fusion with protein-encoding open reading
frames available in ORFeome resources (promoter::ORF::GFP).
Figure 1. Downstream applications of the promoterome clones.
By utilising the cloned C. elegans promterome library, a large number of in vivo assays can be
performed in the nematode. (1) Tissue specific RNAi; (2) Sub-cellular localisation, Co-immunoprecipitation
and Phenotype rescue; (3) Yeast-one-hybrid; (4) ChIP-chip; (5) Expression mapping.
Gateway recombination sites are indicated in: Blue: attB site; Red: attL site; Yellow: attR site.
Format
DNAFORM offers for research purposes only, individual clones from a single well, a
subset of clones or the complete set of 71 plates (96-well format). Re-arrayed
subsets of your chosen clones are available on request.
For further information contact us under: dna@dnaform.jp
References
Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S,
Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M. "A first version of the
Caenorhabditis elegans Promoterome." Genome Res. 2004 Oct;14(10B):2169-75.
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber
Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
