C. elegans has proved invaluable as a worldwide model system for studies in
developmental genetics and neurobiology. The availability of genomic clones
with 35 to 40kb inserts has been a critical resource to the C. elegans
community. Fingerprinted and ordered cosmid clones harbouring such inserts
were the backbone of the genome-sequencing project. Moreover, individual
research labs utilized the clones to map mutants. Complementing a mutant by
injecting a strain with overlapping cosmid clones eventually led to mutation
identity. This approach is still widely used and is an important tool for
mutational analysis. Many of the cosmid libraries are now 20 years old and
there are reports of some of them losing viability.
With these considerations in mind Don Moerman et al have chosen to make a
fosmid rather than a cosmid library. The library was constructed using the
fosmid vector pCC1FOS (Epicentre) and is maintained at low copy number
until induced. The use of fosmids as backbones allows for the maintenance of
large pieces of DNA (around 40kB) in limited number (1-5) per bacterial host.
The N2 fosmid clones are mapped to the C. elegans genome by pair-wise
alignment of fosmid end-reads. Initial analysis of the library quality shows that
the average insert size is ~ 43,284 bp and 86.7% of the clones have paired
end-reads with correct relative orientation. The library obtained approximately
5.74X clone coverage of the genome
Format
DNAFORM offers the complete clone set of 41 384-well microtitre plates or
individual clones, for research purposes only. For further information contact
us under: dna@dnaform.jp
References
Jaryn Perkins1,2, Kim Wong3, Rene Warren3, Jacquie Schein3, Jeff Stott3, Rob
Holt3, Steve Jones3, Marco Marra3, and Don Moerman1,2
- Michael Smith Laboratories, U.B.C., Vancouver, B.C., Canada V6T 1Z4
- Department of Zoology, University of British Columbia, B.C. Vancouver, Canada V6T 1Z4
- Genome Sciences Centre, BC Cancer Agency, Vancouver, B.C. Canada, V5Z 4S6
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