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C. elegans is an invaluable model system for studies in developmental and
neurobiology. In a collaborative effort based at the Dana-Farber Cancer Institute and
Harvard Medical School, Dr Marc Vidal and colleagues have cloned a first version
(version 1.1) of all predicted protein-coding open reading frames (ORFs) of
Caenorhabditis elegans, the so-called "ORFeome". Their effort has resulted in the
generation of over 12,000 clones, of which ~4,000 correspond to genes have not yet
been confirmed by other cDNAs or expressed sequence tag (EST).
These clones offered in an Invitrogen Gateway™ recombinational vector system. As
a starting point, the researchers designed 19,000 primer pairs by computational
methods, based on gene predictions from one of first drafts of the worm genome
annotation made public in 1999. Gene-specific primer pairs would amplify all 'splice
variants' between the two primers designed for any gene. Pools of ~50-1,000
transformants for each set of amplification products were partially sequenced to
produce OSTs (ORF sequence tags). This confirmed gene identity and permitted
identification of pools where at least one processed mRNA was represented. All the
data from this project has been made available in WorfDB, which integrates with
other mapping data (http://worfdb.dfci.harvard.edu/).
Format
DNAFORM offers, for research purposes only, over 10,500 ORF pools from this
collection which can be obtained as individual pooled clones from a single well, a
subset of pools, or the complete set of 114 plates (96-well format). We can also
make subsets of your chosen clones from the collection on request. For further
information contact us under: dna@dnaform.jp
References
Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L,
Bertin N, Janky R, Moore T, Hudson JR Jr, Hartley JL, Brasch MA, Vandenhaute J,
Boulton S, Endress GA, Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek
J, Snyder M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal M. “C.
elegans ORFeome version 1.1: experimental verification of the genome annotation
and resource for proteome-scale protein expression.” Nat Genet. 2003 May;34(1):35-
41.
Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School,
Boston, Massachusetts 02115, USA.
http://vidal.dfci.harvard.edu/
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