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Kazutoyo Osoegawa working in Pieter de Jongs’ laboratory at the Roswell Park Cancer Institute, Buffalo, constructed the mouse PAC library RPCI21. Eirik Frengen constructed the vector pPAC4 used for this library. The source is female 129/SvevTACfBr mouse spleen genomic DNA. The average insert size is 147 Kbp. The library consists of approximately 128,000 clones in 336 384-well-microtitre plates. The plate numbers run from 337 to 672.
Format
The library is available from Source BioScience Ltd as high-density gridded filters for screening by hybridisation. The set consists of 7 double spotted 22.2x22.2 cm filters. For further information contact us under: dna@dnaform.jp.References
- K. Osoegawa et a.l [2000] "Bacterial artificial chromosome libraries for mouse sequencing and functional analysis." Genome Research 10: 116-128
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http://bacpac.chori.org/
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