Full-length cDNA Library

The transcriptome of every cell, tissue and organism is different and there is still a lot more to find beyond what genome sequences can tell us. The best access to the transcriptome of your particular interest is the cloning of all RNA transcripts from your samples into cDNA libraries comprising a cloned copy of each RNA molecule.

DNAFORM's proprietary Full-Length cDNA Library and Cloning Technologies can offer you unmatched solutions for your entry into transcriptome research and beyond.

Full-length cDNA clones and the sequence information derived thereof are the entry to:

• Proteomics : Only full-length cDNAs enable the synthesis, use, and analysis of proteins.
• Gene Regulation : Only full-length cDNAs allow for promoter identification, and as such give you access to the regulatory information that drives gene expression.
• RNAi : The more information you have the greater your chances to prepare a specific RNAi probe.
• Splicing : Only full-length cDNA information can tell you about the true exon composition of transcripts.
• Genomics : Full-length cDNAs are needed for gene discovery and correct mapping of genomic locations.
• SNP Analysis : Only a complete and correct annotation of a gene enables functional characterization of SNPs.

You have the choice:

• Standard Libraries
• Normalized and Subtracted Libraries for Gene Discovery Projects
• Library Subtraction to enrich for the transcripts of your interest
• Pooled Libraries Including Tagging Strategies (combine up to four libraries into one)

Get the Best out of our Services

• Unique full-length rates are the entry to any application you may have.
• Unique redundancy rates enable substantial cost savings in cDNA characterization.
• Unique gene discovery rates open new perspectives in your research.

Whether you need an individual cDNA library as a cloning resource, you are interested in the cloning of differentially expressed genes and functional screens, or you want to build entire cDNA clone collections, DNAFORM will customized cDNA library preparations that to match with your needs.

Approaches in functional genomics depend on high quality libraries to get access to all expressed genes in a given tissue or cell line. Individual clones identified in those libraries can afterwards be studied in detail for their functions and used in various applications. However, those applications depend on full-length cDNA clones encoding the entire proteins. DNAFORM therefore applies patented technologies developed at RIKEN and co-owned by DNAFORM for high standard full-length cDNA cloning.

DNAFORM can prepare Normalized Libraries with increased gene discovery rates. During the Normalization Step the 1st strand cDNA will be hybridized against an aliquot of the initial RNA sample. Due to concentration dependent hybridization kinetics the number clones representing highly expressed genes will be reduced which leads to a more equal distribution of different genes in the library. The normalization approach was optimized for the fast collection of non-redundant clones by random clone picking. DNAFORM can further provide extended normalization/subtraction strategies for large-scale gene discovery projects involving various cDNA libraries.

The cDNA library technology from DNAFORM allows further the preparation of subtracted full-length cDNA libraries for the characterization of e.g. medical conditions. In addition, DNAFORM can perform a normalization step along with the subtraction, which could be useful in cases where one of the two samples will be prepared from cells with a much higher growth rate than the other (e.g. transformed cells versus non-transformed cells). Thus our subtracted libraries allow for the cloning of disease-related genes, which can be further analyzed for their differential expression by other means like DNA Microarrays, RT-PCR, Differential Display, or Northern Blotting.

DNAFORM has developed its on technology for the subtraction of already available cDNA Libraries. This approach may also be applied to re-normalized a cDNA library at a later stage.

As DNAFORM can introduce specific sequence tags during the 1st strand synthesis, we can prepare pooled cDNA libraries that include various samples e.g. as taken at different stages or tissue samples. Commonly such libraries can cover up to 4 different starting materials. Ask about this service if you wish to have highly complex libraries for a wide range of applications and lower costs.

DNAFORM can offer you more than just the cDNA library. Ask for more information on clone isolation, sequencing services, and partnering in genome and transcriptome research.