CAGEキット : 技術情報
□ 技術概要
CAGE(Cap Analysis Gene Expression)法は、遺伝子発現プロファイルやプロモーターを特定する新しいアプローチです。
CAGE法は、完全長cDNAの5'末端にアダプターをライゲーションさせる完全長cDNAライブラリー作製技術がベースになっており、これを次世代シーケンス解析に供します。 CAGEタグをゲノム配列上でマッピングすることにより、転写開始点を同定することができます。 このように、CAGE法は、新規遺伝子の発見、ゲノムワイドな遺伝子発現解析、プロモーター解析の強力なツールとなります。
□他の発現解析技術との比較
遺伝子発現解析の手法は多くあり、それぞれに利点があります。 CAGE法と主な遺伝子発現解析の違いは、CAGE法がゲノムワイドに転写開始点を正確に同定できるという点にあります。
CAGE法では、プロモーター領域の詳細な解析ができるため、特定遺伝子の発現を、制御に関与したプロモーターごとに分けて解析することが可能となり、転写のシグナル伝達のカスケードを明らかにするなど、新しい視点に立ったゲノムアノテーションが可能になります。
CAGE | RNA-seq | SAGE | マイクロアレイ | |
---|---|---|---|---|
未知遺伝子を含む |
○ | ○ | ○ | × |
定量性/ダイナミックレンジ | ◎ ※PCR行程も無く、長さのバイアスも無い |
○ | ○ | △ |
プロモーター部位の同定 | ◎ | △ | × | × |
Transcription Factor Binding Motifの予測 | ◎ | △ | × ※5'末端はデータベース情報に依存 |
× ※5'末端はデータベース情報に依存 |
Bidirectional enhancer RNAの同定 | ◎ | × | × | × |
Alternative Exon1の同定 | ◎ | △ | × | × |
選択的スプライシングや |
× | △ ※Sequence depthによる |
× | × |
全工程にかかる解析所要期間 | △ | △ | △ | ○ |
サンプル調整難易度 | × ※8日間の行程 |
△ | △ | ◎ |
データ解析ツール | △ | ○ | △ | ○ |
□ 論文
□FANTOM主要論文
□その他
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